DU0101-K48 PANEL

DU0101: K48 PANEL (IQF)

$215.00

In stock

SKU: DU-0101-4800 Category: Tags: ,

Specifications

IQF DiUb Technology
LifeSensors’ diubiquitin substrates represent a new class of substrates for the continuous fluorescent measurement of true isopeptidase activity. The C-terminus of wild type ubiquitin is conjugated via an isopeptide bond to lysine 48 (K48) of a second ubiquitin molecule with the resultant diubiquitin forming an internally quenched fluorescent FRET pair (IQF). Each ubiquitin is labeled with a single molecule of either a fluorescent reporter (i.e. TAMRA) or a highly efficient quenching fluorophore. Cleavage of the IQF DiUb by deubiquitylases leads to separation of the fluorophore from quencher and subsequent increase in observed fluorescence.

IQF DiUb Variants
There is a remarkable difference between K48- and K63-linked ubiquitin molecules in their three-dimensional structure, which accounts for their individual functions. Beyond differential linkages (e.g. K48, K63, K11), LifeSensors has created subpanels of IQF diubiquitin substrates within each linkage. Because each DUB is likely to recognize and cleave substrates with unique steric considerations, these subpanels vary in location of reporter fluorophore and quencher. It is recommended that each DUB be empirically evaluated against a panel of IQF DiUb substrates to select the the optimal fluorophore/quencher pairing.In order to rapidly determine which of these fluorophore/quencher pairs are optimal for your DUB and/or determined linkage specificity for your DUB, LifeSensors offers a panel of 6 substrates comprised of each K48-linked substrate available to date. Need bulk quantities? We would be happy to work out a custom price that fits your budget. Please contact us for more information. This product is covered by US patent # 8,518,660. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License found in the product manual.

Info

Species Human
Source E. coli
Tag None
Molecular Weight 18,520.4 +/- 18 Da
Quantity 25 µL of each substrate
Concentration 20 µM
Formulation 50 mM Sodium MES, pH 6.0
Storage +4ºC

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  • Determination of the optimal DiUb48-linked substrate for your DUB
  • Kinetic characterization of deubiquitylases (DUBs)
  • Demonstration of novel DUB activity and/or linkage specificity
  • Monitoring of heterologous DUB production/purification