PE1000A: pE-SUMOpro Amp Kit

The SUMOpro Protein and Peptide Expression Kit maximizes yields of soluble, untagged, functional proteins with the desired N-terminus. While other prokaryotic expression systems use engineered protease cleavage sites that produce amino acid extensions and often result in non-specific cleavage within the protein of interest, LifeSensors’ highly active and specific SUMO protease 1 recognizes the tertiary structure of the SUMO tag. This generates the desired N-terminus and never cleaves within your protein of interest. The SUMOpro kit makes purifying your protein affordable and straightforward. By linking a His6 epitope to both the SUMO fusion tag and SUMO Protease 1, the SUMOpro system allows efficient and rapid purification of untagged protein using immobilized metal affinity chromatography. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Info

Kit Components: -pE-SUMO Amp vector (Cat.# 1001A)- 20 µg -SUMO Protease 1 (Cat.# 4010)-500 Units -SUMO Protease control protein (Cat.# 5000) – 100 µg -Anti-SUMO/SUMOstar antibody (Cat.# AB7002) – 50 µg

Fusion tag	His6-SUMO
Quantity	n/a
Promoter	T7 RNA polymerase
Selection marker	Ampicillin (E. coli)
Host	E. coli
Storage	Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.