Specifications
The SUMOpro Protein and Peptide Expression Kit maximizes yields of soluble, untagged, functional proteins with the desired N-terminus. While other prokaryotic expression systems use engineered protease cleavage sites that produce amino acid extensions and often result in non-specific cleavage within the protein of interest, LifeSensors’ highly active and specific SUMO protease 1 recognizes the tertiary structure of the SUMO tag. This generates the desired N-terminus and never cleaves within your protein of interest. The SUMOpro kit makes purifying your protein affordable and straightforward. By linking a His6 epitope to both the SUMO fusion tag and SUMO Protease 1, the SUMOpro system allows efficient and rapid purification of untagged protein using immobilized metal affinity chromatography.
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Info
Kit Components:
-pE-SUMO Amp vector (Cat.# 1001A)- 20 µg
-SUMO Protease 1 (Cat.# 4010)-500 Units
-SUMO Protease control protein (Cat.# 5000) – 100 µg
-Anti-SUMO/SUMOstar antibody (Cat.# AB7002) – 50 µg
| Fusion tag | His6-SUMO |
| Quantity | n/a |
| Promoter | T7 RNA polymerase |
| Selection marker | Ampicillin (E. coli) |
| Host | E. coli |
| Storage | Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles. |
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