LifeSensors pioneered the application of SUMO system to enhance production of polypeptides as published by Marblestone et. Al, Protein Sci. 2006. SUMO system is superior to several other tags: 6xHis, Ubiquitin, MBP, GST, and TRX. More recent studies [1],[2],[3],[4],[5] have shown a range of applications through expression of antimicrobial, cytotoxic peptides, and anti-thrombotic peptides.

Removal of the polypeptide from the tag is costly or impossible due to the inability of the proteases to cleave and provide the desired amino acid sequence. LifeSensors’ SUMO proteases recognize the SUMO structure and does not discriminate between fused polypeptide cleavage sites by generating the desired N-terminal sequence. As shown in the table below (Table 1), the cost of enzymatic cleavage of polypeptide is a major factor that has been a hurdle in adopting recombinant methods for polypeptide production. LifeSensors has developed the most efficient SUMO protease to reduce the cost of goods. As shown in the table below, SUMO mediated manufacturing of the polypeptide is the most cost-effective method. We encourage inquiries to establish cost-effective pilot scale manufacturing of polypeptides. The manufacturing process can be seamlessly transferred to GMP facilities.