Polypeptide Manufacturing

Advanced SUMO technology for cost-effective polypeptide production

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Polypeptides are commonly synthesized by traditional chemistry. There are a few disadvantages of chemical synthesis of polypeptides:

SUMO (Small Ubiquitin-like Modifier) is a member of the ubiquitin family, composed of a flexible N-terminal region followed by a ball-shaped ubiquitin-like fold.

Hydrophobic Core

Improves correct folding of the fused polypeptides

Hydrophilic Surface

Keeps the partner protein soluble

Precise Cleavage

SUMO protease recognizes SUMO structure to cleave at the junction

Increased Yield

Stabilize polypeptide to increase the yield significantly

Desired N-Terminal

Encode desired N-terminal sequence with precision

Correct Folding

Ensure correctly folded polypeptide due to SUMO chaperoning properties

SUMO system is the most cost-effective way to manufacture growth hormones, glucagon-like polypeptides, and antimicrobial and industrial polypeptides.

Contact LifeSensors to decrease the cost of goods for your polypeptide production. We will guide you about the best way to manufacture your polypeptide and transfer the manufacturing process to GMP sites.

SUMO (Small Ubiquitin-like Modifier) is a member of the ubiquitin family, composed of a flexible N-terminal region followed by a ball-shaped ubiquitin-like fold.

Expression

SUMO tag enhances polypeptide expression

Purification

6xHis tag assists in purification process

Cleavage

SUMO protease releases polypeptide

LifeSensors pioneered the application of SUMO system to enhance production of polypeptides as published by Marblestone et. al, Protein Sci. 2006. SUMO system is superior to several other tags: 6xHis, Ubiquitin, MBP, GST, and TRX.

Recent studies have shown a range of applications through expression of:

Unique Advantage

Removal of the polypeptide from the tag is costly or impossible due to the inability of the proteases to cleave and provide the desired amino acid sequence.

LifeSensors' SUMO proteases recognize the SUMO structure and does not discriminate between fused polypeptide cleavage sites by generating the desired N-terminal sequence.

As shown in the table below, the cost of enzymatic cleavage of polypeptide is a major factor that has been a hurdle in adopting recombinant methods for polypeptide production. LifeSensors has developed the most efficient SUMO protease to reduce the cost of goods.

We encourage inquiries to establish cost-effective pilot scale manufacturing of polypeptides. The manufacturing process can be seamlessly transferred to GMP facilities.

Peptide Production Presentation

Preview our comprehensive presentation on peptide production techniques

To use, simply utilize the drop-down arrows in order to search for your target of interest or application category. LifeSensors SUMO technology has been utilized for the expression of many different targets and continues to be utilized worldwide.