The LifeSensors’ UbiQuant S ELISA is an industry-leading platforms for measuring ubiquitylation of a target protein in cells. This assay utilizes TUBEs (Tandem Ubiquitin Binding Entities) to detect ubiquitylated substrates, and are individually optimized for a specific substrate protein.
Conceptual schematic of the UbiQuant S ELISA assay. Polyubiquitlyated proteins are captured by an immobilized ubiquitin affinity matrix. Subsequently, unbound proteins are washed away, and ubiquitylated protein of interest bound to the matrix is identified using a POI-specific antibody and traditional ELISA detection reagents.
Sample data from UbiQuant S ELISA and AlphaLISA assays. Jurkat cells were treated with bortezomib (BTZ) or BTZ in combination with selective USP7 inhibitor P0217564 (564). Following treatment and cell lysis, Ubiquitylation of USP7 was measured by the UbiQuant S ELISA and AlphaLISA assays.