UM606: M1 TUBE (FLAG)

LifeSensors UM606 is an Anti-M1 (linear) Tandem Ubiquitin Binding Entity (TUBE) for high-affinity isolation and enrichment of M1-polyubiquitinated proteins in research applications.

$707.00

SKU UM-0606-0050 Categories M1 TUBE1, TUBEs: Ubiquitin Affinity Matrix Tags Linkage-specific, Pull down, Tandem Ubiquitin Binding entities

In stock

Product Advantages

Applications

Isolation and enrichment of M1-polyubiquitinated proteins from cell and tissue extracts

Isolation of ubiquitylated proteins for proteomic studies

Benefits

Nanomolar dissociation constant (Kd) for M1-chains

1000 to 10,000-fold preference for M1 chains over K48- or K63- chains

Overexpression of epitope-tagged ubiquitin for pull downs is not necessary

Compatible with Ni+2IMAC, streptavidin, or FLAG® -based isolation technologies, providing flexibility and specificity

LifeSensors has developed TUBE (Tandem Ubiquitin Binding Entity) technology for the detection, characterization and isolation of polyubiquitylated proteins from cells and tissue extracts. TUBEs capitalize on the linkage of multiple ubiquitin interacting motifs (UIMs) to generate reagents with high affinity for polyubiquitin.TUBEs have 100 to 1000-fold higher affinity for polyubiquitin chains compared to monomer ubiquitin binding domains (UBDs). TUBEs both stabilize and bind to ubiquitylated proteins, serving as an indispensable tool for ubiquitologists. Our versatile collection of TUBEs includes reagents for the isolation/pulldown of polyubiquitin conjugates include both linkage-specific and non-specific reagents including M1 (Linear) TUBE, K63 TUBE, K48 TUBE, and the non-specific reagents TUBE 1 and TUBE 2. This product is protected by one or more US or Foreign patents.