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Questions about a product, technology or experimental design? Give us a call 610-644-8845.

UM400: Control Agarose Beads (negative control)

LifeSensors UM400 are control uncoupled agarose beads used as a negative control in ubiquitin-related assays, ensuring specificity in protein ubiquitination studies.

$92.00

In stock

In stock

Control Agarose Beads. For use in “pre-clearing” cell lysates, as well as a negative control for the pulldown of polyubiquitylated proteins in conjunction with agarose-conjugated TUBEs (UM401 and UM402). Control Agarose is generated by blocking an activated matrix (identical to that used for TUBEs conjugation) with ethanolamine. Control Agarose (200 µl resin) is supplied as a 50% slurry in PBS, pH 7.2, 20% ethanol.

Info

Tag

N/A

Molecular Weight

Physical State

Liquid

Quantity

400 µL

Concentration

Variable

Storage

-20°C, avoid freeze/thaw cycles, avoid storing below this temperature

This product has been cited 1 time.

Applications

  • “Pre-clearing” of cell lysates prior to pulldown experiments
  • Negative control for pull-down experiments
  • General recommendations: pre-clear 1ml of cell lysate (3-5mg/ml total protein) through incubation with 30µl resin for 1hr (4°C). Individual conditions may have to optimized by the end user.

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

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