UM400: Control Agarose Beads (negative control)

LifeSensors UM400 are control uncoupled agarose beads used as a negative control in ubiquitin-related assays, ensuring specificity in protein ubiquitination studies.

$115.00

SKU UM-0400-0400 Categories Pan-Selective TUBEs, TUBEs: Ubiquitin Affinity Matrix

In stock

Product Advantages

Applications

“Pre-clearing” of cell lysates prior to pulldown experiments
Negative control for pull-down experiments
General recommendations: pre-clear 1ml of cell lysate (3-5mg/ml total protein) through incubation with 30µl resin for 1hr (4°C). Individual conditions may have to optimized by the end user.

Control Agarose Beads. For use in “pre-clearing” cell lysates, as well as a negative control for the pulldown of polyubiquitylated proteins in conjunction with agarose-conjugated TUBEs (UM401 and UM402). Control Agarose is generated by blocking an activated matrix (identical to that used for TUBEs conjugation) with ethanolamine. Control Agarose (200 µl resin) is supplied as a 50% slurry in PBS, pH 7.2, 20% ethanol.

Info

Tag	N/A
Molecular Weight
Physical State	Liquid
Quantity	400 µL
Concentration	Variable
Storage	-20°C, avoid freeze/thaw cycles, avoid storing below this temperature

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Datasheet
SDS

Datasheet
SDS

Applications

“Pre-clearing” of cell lysates prior to pulldown experiments
Negative control for pull-down experiments
General recommendations: pre-clear 1ml of cell lysate (3-5mg/ml total protein) through incubation with 30µl resin for 1hr (4°C). Individual conditions may have to optimized by the end user.