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SP4110: SUMOstar Protease

LifeSensors SP4110 is a recombinant SUMOstar Protease that specifically cleaves SUMOstar tags from fusion proteins, enabling efficient protein purification and research applications.

$459.00 – $6,104.00

SKU SP-4110-0250 Categories , ,
  • SUMOstar Protease 1 contains a polyhistidine tag at the N-terminus, allowing easy removal from the cleavage reaction by affinity chromatography. The protease cleaves consistently over a broad range of temperature (30°C is optimal), pH [5.5-9.5], and ionic strength.
  • Specifically engineered to recognize and cleave the SUMOstar fusion tag
Recombinant SUMOstar Protease 1 specifically recognizes and cleaves the SUMOstar tag from recombinant fusion proteins made with SUMOstar expression systems (Cat.#s: 1100A, 1106, 1100K, 1101, 2100, 2101, 2160, 2161, 3100, 3101, 7110, 7111, 7120, 7121). Unlike thrombin, EK, or TEV proteases, which recognize short, linear sequences, SUMOstar Protease 1 recognizes the tertiary structure of SUMOstar. As a result, SUMOstar Protease 1 will not cleave within the protein of interest. SUMOstar Protease 1 will not cleave SUMO-tags from proteins expressed with the SUMOpro or SUMOpro3 systems. To cleave the SUMOpro tag (orginial yeast SUMO tag), please use SUMO Protease 1 and to cleave SUMOpro3 tag please use SUMO Protease 2. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License. Need bulk quantities? We would be happy to work out a custom price that fits your budget. Please click here to request a quote.

Info

Species Yeast
Source E. coli
Tag His6 
Molecular Weight 26.4 kDa
Quantity 250, 500, 1000, 5000 units
Concentration Variable
Formulation 50 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles
  • SUMOstar Protease 1 contains a polyhistidine tag at the N-terminus, allowing easy removal from the cleavage reaction by affinity chromatography. The protease cleaves consistently over a broad range of temperature (30°C is optimal), pH [5.5-9.5], and ionic strength.
  • Specifically engineered to recognize and cleave the SUMOstar fusion tag

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