SI6304B: Biotinylated K63-Linked Tetra-Ubiquitin

K63 chains traditionally play a role in intracellular signaling, trafficking, and autophagy. The topology of this linkage and its apparent role in cellular processes are quite different from that of K48. Poly-Ub chains of this type appear to play a role in endocytic trafficking, DNA repair, neurodegeneration and more. K63 ubiquitin is formed via an isopeptide bond between Lysine 63 and the C-terminal Glycine. This linkage takes place onto other ubiquitin monomers and are then enzymatically linked from specific UPS enzymes. Once the K63 Tetra Ubiquitin has been formed we then biotinylate it once on one available site on the ubiquitin chain. This biotin then acts as a means of detection via streptavidin or avidin. The applications are numerous from Western Blots to ELISAs. Testing can vary based off intended application (EX: DUB activity/specificity).   Info

Species	Human
Source	E. coli
Tag	Biotin
Molecular Weight	34759 Da (depending on degree of phosphorylation)
Quantity	25µg
Concentration	Variable
Formulation	20 mM Tris pH 7.5, 150 mM NaCl
Storage	-80°C, avoid freeze/thaw cycles