SI520: hSUMO2-AMC

$200.00

In stock

SKU: SI-0560-0050 Category: Tag:

Specifications

Human SUMO2-AMC is prepared via the conjugation of the C-terminus of mature human Small Ubiquitin-like Modifier (SUMO)2 to 7-amino-4-methylcoumarin (AMC), leading to quenching of the intrinsic fluorescence of AMC. Upon incubation with desumoylases, AMC is released and an increase in fluorescence can be measured using at 460 nm (excitation at 380 nm). The high purity of LifeSensors’ SUMO2-AMC results in improved to signal:background ratios. Optimization of assay parameters and reagent requirements must be determined by the end user.

Info

Species Human
Source E. coli
Tag None
Molecular Weight 10,723 Da
Quantity 50 µg
Concentration Variable
Formulation Lyophilized powder
Storage -80°C, avoid freeze/thaw cycles

Additional information

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Applications

  • Determining the activity and specificity of isopeptidases for Ub/Ubl proteins.
  • High Througput Screening for modulators of dSUMOylase activity.

Benefits

  • Highly sensitive compared to hSUMO2-AMC, hSUMO2-Rho110, and Z-RLRDGG-aminoluciferin
  • Allows for assaying activity at lower enzyme concentrations
  • Is active with SENP1, SENP2, and SENP6

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