SI520: hSUMO2-AMC

LifeSensors SI520 is a highly sensitive hSUMO2-AMC substrate for detecting SUMO protease activity at low enzyme concentrations, ideal for precise biochemical assays.

$521.00

SKU SI-0520-0050 Category Sumo Derivatives Chain

In stock

Product Advantages

Applications

Determining the activity and specificity of isopeptidases for Ub/Ubl proteins.
High Througput Screening for modulators of dSUMOylase activity.

Benefits

Highly sensitive compared to hSUMO2-AMC, hSUMO2-Rho110, and Z-RLRDGG-aminoluciferin
Allows for assaying activity at lower enzyme concentrations
Is active with SENP1, SENP2, and SENP6

Human SUMO2-AMC is prepared via the conjugation of the C-terminus of mature human Small Ubiquitin-like Modifier (SUMO)2 to 7-amino-4-methylcoumarin (AMC), leading to quenching of the intrinsic fluorescence of AMC. Upon incubation with desumoylases, AMC is released and an increase in fluorescence can be measured using at 460 nm (excitation at 380 nm). The high purity of LifeSensors’ SUMO2-AMC results in improved to signal:background ratios. Optimization of assay parameters and reagent requirements must be determined by the end user.

Info

Species	Human
Source	E. coli
Tag	None
Molecular Weight	10,723 Da
Quantity	50 µg
Concentration	Variable
Formulation	Lyophilized powder
Storage	-80°C, avoid freeze/thaw cycles

Document 1

Document 1

Applications

Determining the activity and specificity of isopeptidases for Ub/Ubl proteins.
High Througput Screening for modulators of dSUMOylase activity.

Benefits

Highly sensitive compared to hSUMO2-AMC, hSUMO2-Rho110, and Z-RLRDGG-aminoluciferin
Allows for assaying activity at lower enzyme concentrations
Is active with SENP1, SENP2, and SENP6