SI4804BP: Biotinylated K48-Linked Tetra-Ubiquitin (Phosphorylated)

K48 chains traditionally play a role in proteasomal degradation pathways. These polymeric chains of ubiquitin molecules play critical roles in regulating protein stability, localization, and activity. K48 chains traditionally play a role in proteasomal degradation pathways and have a central role in mitophagy signaling pathways. K48 linkage remains a critical pathway for the cells to maintain homeostasis through proteolytic degradation, and as such remains very intriguing for the study of DUBs that play a role in the degradation, as well as the proteasome itself. Phospho-ubiquitin chains represent a specialized class of polyubiquitin characterized by phosphorylation at Serine 65 and play a central role in mitophagy signaling pathways. K48 Tetra-Ubiquitin (phosphorylated) is a tetrameric chain of wild-type ubiquitin, wherein ubiquitin monomers are linked together via an isopeptide bond between Lysine 48 and the C-terminal Glycine. The chains are then enzymatically phosphorylated at the Ser65 position. Once the K48 Tetra-Ubiquitin has been formed and phosphorylated we then biotinylate it once on one available site on the ubiquitin chain. This biotin then acts as a means of detection via streptavidin or avidin. The applications are numerous from Western Blots to ELISAs. Testing can vary based off of intended application (EX: DUB activity/specificity). Info Species Human Source E. coli Tag Biotin Molecular Weight 34842-35082 Da (depending on degree of phosphorylation) Quantity 25µg Concentration Variable Formulation 20 mM Tris pH 7.5, 150 mM NaCl Storage -80°C, avoid freeze/thaw cycles