CL100: HeLa WT Lysate Control Panel to Study Mitophagy

Specifications

HeLa cells are an immortalized line of human epithelial cells taken from the uterus and cervix of Henrietta Lacks, a patient with adenocarcinoma. CCCP (carbonyl cyanide m-chlorophenyl hydrazone) disrupts mitochondrial membrane potential and leads its depolarization and dysfunction of the mitochondria. Cells treated with CCCP treated cells can be used in phosphoubiquitination studies because it induces mitophagy and the accumulation of phosphorylated mitochondrial proteins. The lysate is available both with and without treatment with proteasome inhibitor MG-132, the mitochondrial uncoupler CCCP, and the macrolide antibiotic bafilomycin. Cell pellets were lysed using RIPA complete with proteasome, phosphatase, and DUB inhibitors.

This kit consists of HeLa WT Lysate treated with:

• DMSO
• CCCP 10µm
• DMSO, MG132, Bafilomycin
• CCCP, MG132, Bafilomycin.