In eukaryotes, endogenous SUMO proteases rapidly cleave SUMO fusions in vivo, precluding tag-enhanced expression and purification capacities. To circumvent this problem, LifeSensors has engineered the SUMOstar fusion system. SUMOstar offers the same solubility and expression enhancing benefits as the original wild-type SUMO tag; however, the tag is not cleaved by endogenous eukaryotic SUMO proteases. In addition, LifeSensors has created a cognate SUMOstar protease for specific recognition and cleavage of the SUMOstar tag in vitro, liberating the protein-of-interest with a desired N-terminal amino acid. The pM-Mammalian Intracellular SUMOstar Kit allows for expression of SUMOstar fusion proteins intracellularly.
This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply with these terms.
|Selection marker||Ampicillin (E. coli), G418 (mammalian)|
|Storage||Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.|