![](https://lifesensors.com/wp-content/uploads/2019/09/elisa2.jpg)
The ELISA assay is based on the capture of the polyubiquitinated E3 ligase substrate by a proprietary polyubiquitin binding reagent. The polyubiquitinated substrate is detected using chemiluminescent-conjugated TUBEs. The chemiluminescent signal can be followed over time in a homogenous, high-throughput format, making it ideal for small-molecule screening.
![](https://lifesensors.com/wp-content/uploads/2019/09/fret-e3-data2.jpg)