SI4804B: Biotinylated K48-Linked Tetra-Ubiquitin

K48 chains traditionally play a role in proteasomal degradation pathways. These polymeric chains of ubiquitin molecules play critical roles in regulating protein stability, localization, and activity. K48 chains traditionally play a role in proteasomal degradation pathways and have a central role in mitophagy signaling pathways. K48 linkage remains a critical pathway for the cells to maintain homeostasis through proteolytic degradation, and as such remains very intriguing for the study of DUBs that play a role in the degradation, as well as the proteasome itself. K48 Tetra-Ubiquitin is a tetrameric chain of wild-type ubiquitin, wherein ubiquitin monomers are enzymatically linked together via an isopeptide bond between Lysine 48 and the C-terminal Glycine. Once the K48 Tetra-Ubiquitin has been formed and phosphorylated it is then biotinylated once on one available site on the ubiquitin chain. This biotin then acts as a means of detection via streptavidin or avidin. The applications are numerous from Western Blots to ELISAs.  

Species	Human
Source	E. coli
Tag	Biotin
Molecular Weight	35207 Da (depending on degree of phosphorylation)
Quantity	25µg
Concentration	Variable
Formulation	20 mM Tris pH 7.5, 150 mM NaCl
Storage	-80°C, avoid freeze/thaw cycles