SI270A: Ubiquitin, ALEXA-labeled

Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the ε-amino group of a lysine (Lys) residue on the target substrate [1]. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains [2, 3]. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, LifeSensors’ Alexa-labeled ubiquitin carries a single Alexa-647 molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.

Info

Purity	> 95%
Solubility	> 8mg/mL
Molecular Weight	9562 Da
Quantity	25 µg
Physical State	Liquid, PBS
Wavelength Maxima	Ex: 651 nm, Em: 667 nm
Storage	-80°C, avoid freeze/thaw cycles