UbiTest Substrate Validation
Advanced platform for measuring polyubiquitylation levels using innovative TUBE technology
Need Help?
- Overview
LifeSensors’ UbiTest is a medium-throughput platform for measuring the polyubiquitylation levels of protein(s) of interest (POI). UbiTest utilizes TUBE technology to enrich polyubiquitylated proteins. This fraction is analyzed by immunoblotting techniques with and without pan-selective DUB digestion. The appearance or increase in the intensity of the band corresponding to the POI indicates that the POI is polyubiquitylated in the studied condition. Identification of the type of ubiquitin linkage can provide insights into the cellular functions of E3s and substrates.
- Service Highlights
Cell-Based Assays
Cell-based assays to measure endogenous substrate ubiquitination.
Simplified Quantification
Conversion of polyubiquitin smear of the POI to a single band in the DUB-treated fraction simplifies the quantification.
Overcome Detection Issues
Overcome the issue of target-specific primary antibody failing to detect ubiquitinated target protein due to epitope masking by the bulky ubiquitin chain.
Linkage Identification
Identify the polyubiquitin linkages on the POI by digesting with linkage-specific DUBs.
Study Small Molecules
Study the effect of PROTACs or small molecules.
- Resources
E3 Ligase Profiling & Screening Services
Download our comprehensive guide to learn more about our services
- Products
- DUBs
Related products are displaying below
Product / Tools Name
SKU
Categories
Add to cart
- VU1 Products
Related products are showing below