VU101H: Anti-Ubiquitin, HRP-Conjugated (MAb; clone VU-1)

$285.00

In stock

SKU: VU-0101H-0050 Categories: ,

Specifications

LifeSensors has developed several monoclonal antibodies that recognize polyubiquitylated proteins and free ubiquitin. VU-1 recognizes all ubiquitin linkages (mono, K6, K11, K27, K29, K33, K48, K63, and linear) and now we have conjugated VU-1 to HRP so western blotting is performed in a single step (no secondary antibody required). Ubiquitin (Ub), a highly conserved, 8 kDa polypeptide present in all eukaryotic cells, is conjugated to the e-amino group of lysine residues in the target protein through the sequential action of three enzymes, an E1 Ub activating enzyme, an E2 conjugating enzyme, and an E3 ligase. In addition, the seven lysines within Ub itself can serve as Ub acceptors leading to the formation of polyubiquitin chains. Among these, K48 and K63 linkages are well characterized; K48-linked polyubiquitylation targets proteins for proteasome degradation whereas K63 linkages regulate signaling events, receptor endocytosis and immune responses. Polyubiquitin linkages at other lysines are less prominent and their physiological roles are under investigation.

Info

Host Mouse
Quantity 50 µg
Concentration Variable
Storage -20°C; Avoid freeze/thaw cycles

Additional information

SKU

Reviews

There are no reviews yet.

Be the first to review “VU101H: Anti-Ubiquitin, HRP-Conjugated (MAb; clone VU-1)”
  • Western blots: 1:2000 dilution
  • To obtain maximum sensitivity, the membrane should be treated with 0.5% glutaraldehyde prior to antibody exposure
  • Western blot analysis to detect all types of ubiquitin linkages (mono, K6, K11, K27, K29, K33, K48, K63, and linear)