UbiQuant™ is a sandwich assay that allows for the absolute measurement of ubiquitin in a cell lysate. This plate-based assay relies on capturing total ubiquitin using a proprietary ubiquitin binding reagent. The enzyme-linked anti-ubiquitin monoclonal (VU-2) antibody generates a signal that assessed by a plate reader. The use of a ubiquitin standard curve provides a direct measurement of ubiquitin concentration.
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