“The time resolved FRET assay for E1 ternary complex formation represents a significant advance in speed and utility that should be particularly useful for those interested in routine quality control measurements, high throughput activity screens, or rapid reaction kinetics for which isotopic methods are precluded.”
– Arthur L. Haas, Ph.D.
Roland Coulson Professor and Head
Department of Biochemistry and Molecular Biology
LSU Health Sciences Center School of Medicine
My laboratory has published two recent manuscripts reporting the use of FLAG-K63 and FLAG-K48 TUBE reagents. We used these reagents to study the ubiquitination of the nonstructual 2 transmembrane protein of hepatitis C virus (HCV) by the cellular E3 ligase MARCH8. By being quite selective and easy to handle, these reagents helped us characterize the specific ubiquitin modification.
My lab has successfully used the agarose-TUBEs. LifeSensors’ TUBEs are easy to use and highly specific. My lab is looking forward to using the linkage specific TUBEs.
We have used several LifeSensors products to investigate the role of the ubiquitin pathway in human cancer cells. I have been extremely impressed with the quality and reproducibility of their products. Their technical department are knowledgeable and their prompt assistance always enabled us to troubleshoot practical issues for diverse set of experiments.
I am so pleased to let you know that my group and, as a consequence of our positive experience with your products, also many of my collaborators have successfully used the high-quality ubiquitin-related reagents provided by LifeSensors!
Fusing sumo to my protein made a tremendous difference in expression level of my proteins. I went from using WB to detect it to being able to see it on a gel stained with coomasie. LifeSensors’ SUMO Protease 1 is the best and most robust enzyme among SUMO proteases offered by other vendors. It’s consistent and does not cause proteolitic degradation. I see a dramatic increase in the quality and quantity of my protein.
The TUBE products from LifeSensors are a valuable set of tools for any lab investigating ubiquitin post-translational modifications. Their reagents make it easy to look at ubiquitin post-translational modifications on our proteins of interest. We use the TUBE products to immunoprecipitate ubiquitylated proteins from cell lysates and then perform a Western blot. Since we can use specific antibodies that we have previously optimized for Western blot, there are no additional optimization steps and the results look great. I would definitely recommend the TUBE products from LifeSensors to other investigators looking for a fast and economical way to look at specific ubiquitin post-translational modifications.
We consistently use your products because of the quality and purity in comparison to other companies and because your scientists take the time to help us troubleshoot technical obstacles we encounter in our research.
We have tried the SUMOStar technology on one of the toughest proteins in our mammalian expression platform portfolio, which previously failed to express in various expression vectors in HEK293 cells. The SUMO fusion approach resulted in an astounding yield of 3 mg of the purified recombinant protein per Litre. Due to the conformation of the fusion protein (it was a tight dimer), we experienced difficulties when trying to remove the SUMO tag. We therefore contacted the Lifesensors’ Technical Support. The feedback was remarkably speedy, expertly and very helpful: our problem was resolved. Excellent technology. Excellent product support.
The VU-1 antibody from LifeSensors is the best anti-ubiquitin antibody that we have found after trying more than ten different anti-ubiquitin antibodies. Please click here for his paper comparing VU-1 to other antibodies.