UM900M: High Capacity Sample Panel

Specifications

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” protein in its polyubiquitinated state. TUBEs can be used to isolate, enrich, and identify ubiquitinated proteins from cell and tissue extracts. Magnetic beads coupled to TUBEs allow for efficient recovery of polyubiquitinated proteins in a single step without centrifuge. The magnetic TUBEs allows more complete removal of the unbound supernatant, which leads to lower background. Polyubiquitinated proteins can easily be separated from TUBEs for proteomic studies or identification by Western blotting.

This sample panel consists of a comprehensive TUBE suite, to test which TUBE is most suitable to your personal application.

Includes:

UM500M – High Capacity Magnetic Control Beads

UM501M – High Capacity Magnetic TUBE1

UM502M – High Capacity Magnetic TUBE2

UM407M – K48 TUBE HF (Magnetic Beads)

UM404M – K63 TUBE (Magnetic Beads)

UM406M – M1 Linear Ubiquitin TUBE (Magnetic)

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