LifeSensors’ UbiQuant ELISA assay that allows for determining the concentration of total ubiquitin in cell and tissue lysates. Ubiquitylated proteins are captured by an immobilized ubiquitin affinity matrix. Subsequently, unbound proteins are washed away, and total free ubiquitin and ubiquitin conjugates are quantified using an anti-ubiquitin antibody in conjunction with traditional ELISA detection reagents.
Advantages:
- UbiQuant ELISA allows for the testing of the effect of multiple compounds or conditions on the ubiquitylation levels in cell/tissue lysates.
- This assay is useful for monitoring the effects of broad-spectrum compounds such as proteasome inhibitors on total ubiquitin levels.