UC105: E1 LITE – UBE1 Activity Assay Kit

SKU UC-0105 Categories E1-E2-E3 Assays, Kits and Plates, Research Kits, Ubiquitin Ligases and Enzymes

LifeSensors UC105 is the E1 LITE UBE1 Activity Assay Kit designed for sensitive detection of UBE1 enzyme activity, supporting research in ubiquitination pathways.

$663.00

Plate Size(*)

Clear

Plate Size(*)

Clear

The E1 LITE – UBE1 Activity Assay is a kit for assessing E1 enzyme activity. This plate-based assay is based on the transfer of FRET energy between the acceptor-charged ubiquitin and the donor-charged ubiquitin as they both bind to the E1 enzyme. The assay employs terbium as the donor fluorophore, which has a long-lived fluorescence allowing for a time-delayed reading of the FRET signal, or time-resolved FRET (TR-FRET). This reduces the contribution from buffers, proteins, and chemical compounds. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Manual
License

Applications

Screening for inhibitors of the ubiquitin E1 enzyme, UBE1
Characterization of UBE1 kinetics
Characterization of the mechanisms of E1 inhibitors
Secondary screening of E3 ligase, kinase, or other target inhibitors for inhibition of UBE1
Eliminates the need for radioactive or Western blot-based detection of E1-ubiquitin-thioester formation
Homogenous assay compatible with high-throughput screening for UBE1 inhibitors
Compatible with 96-well and 384-well formats

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

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UC105: E1 LITE – UBE1 Activity Assay Kit

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