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UC105: E1 LITE – UBE1 Activity Assay Kit
LifeSensors UC105 is the E1 LITE UBE1 Activity Assay Kit designed for sensitive detection of UBE1 enzyme activity, supporting research in ubiquitination pathways.
Screening for inhibitors of the ubiquitin E1 enzyme, UBE1
Characterization of UBE1 kinetics
Characterization of the mechanisms of E1 inhibitors
Secondary screening of E3 ligase, kinase, or other target inhibitors for inhibition of UBE1
Eliminates the need for radioactive or Western blot-based detection of E1-ubiquitin-thioester formation
Homogenous assay compatible with high-throughput screening for UBE1 inhibitors
Compatible with 96-well and 384-well formats
The E1 LITE – UBE1 Activity Assay is a kit for assessing E1 enzyme activity. This plate-based assay is based on the transfer of FRET energy between the acceptor-charged ubiquitin and the donor-charged ubiquitin as they both bind to the E1 enzyme. The assay employs terbium as the donor fluorophore, which has a long-lived fluorescence allowing for a time-delayed reading of the FRET signal, or time-resolved FRET (TR-FRET). This reduces the contribution from buffers, proteins, and chemical compounds.
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