UB206: MMS2 (yeast)

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Specifications

MMS2 is an ubiquitin E2 variant (UEV) protein that acts in concert with the E2 enzyme Ubc13. This heterodimeric complex assembles K63-linked polyubiquitin chains for non-degradative ubiquitin signaling pathways (DNA repair, signal transduction). The UEV proteins are structurally similar to E2 enzymes; they display the same fold, yet lack the active site cysteine indicative of E2 enzymes. The Ubc13/MMS2 complex is unique in that it has the ability to form free K63-linked polyubiquitin chains in solution in the absence of an E3.

Info

Species S. cerevisiae
Source E. coli
Tag None
Molecular Weight 15,544.7 Da
Quantity 0.8 nmol, 3.0 nmol
Concentration 40 µM
Formulation 50 mM Tris pH 7.5, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Nanomole

0.8 nmol, 3 nmol

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  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

Benefits

  • Untagged (native) E2 conjugation enzyme