UB223H: UBE2W, His6

LifeSensors UB223H is a His6-tagged UBE2W E2 ubiquitin-conjugating enzyme, essential for studying ubiquitination pathways and protein regulation in research.

$210.00 – $417.00

SKU UB-223H Categories E2 Ubiquitin Conjugating Enzymes, Ubiquitin Ligases and Enzymes

Product Advantages

Applications

Identify cognate E2/E3 pairs
Characterize upregulation/downregulation of E2 enzymes
Build poly-ubiquitin chains
Characterize ubiquitin chain specificity of E2 enzymes
Analyze expression of E2 enzymes

UBE2W is a ubiquitin conjugating enzyme that mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase. UBE2W was originally identified as the human homolog of the Arabidopsis E2 enzyme Ubc-16. Researchers demonstrated that UBE2W contains a NLS sequence and is localized to the nucleus of HEK293 cells. UBE2W binds to the E3 ligase BRCA1 and mediates the monoubiquitylation of BRCA12. In vitro UBE2W catalyzes monoubiquitination and ‘Lys-11’-linked polyubiquitination.

Info

Species	Human
Source	E. coli
Tag	His₆
Molecular Weight	18,456 Da
Quantity	0.8 nmol, 3.0 nmol
Concentration	40µM
Formulation	TBS, 10% glycerol
Storage	-80°C, avoid freeze/thaw cycles