The E1/E2 LITE – Ubiquitin Transfer Assay is a kit for assessing E1/E2 ubiquitin transfer activity. This plate-based assay is based on the transfer of FRET energy between the acceptor-charged ubiquitin to the donor-charged E2 enzyme upon binding of the two. The assay employs terbium as the donor fluorophore, which has a long-lived fluorescence allowing for a time-delayed reading of the FRET signal, or time-resolved FRET (TR-FRET). This reduces the contribution from buffers, proteins, and chemical compounds.
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