UM401: TUBE 1 (Agarose)

LifeSensors UM401 is an agarose-coupled Tandem Ubiquitin Binding Entity (TUBE 1) that efficiently captures polyubiquitinated proteins for proteomic and Western blot analysis.

$1,002.00

SKU UM-0401-1000 Categories Pan-Selective TUBEs, TUBEs: Ubiquitin Affinity Matrix Tags Pan-selective, Pull down, Tandem Ubiquitin Binding entities

In stock

Product Advantages

Applications

Pull down of polyubiquitylated proteins from cell lines, tissues and organs

Isolation of polyubiquitinated proteins for proteomic studies

Protection of polyubiquitylated proteins from deubiquitylation and degradation by the proteasome

Benefits

One step recovery of polyubiquitinated proteins

Efficient isolation of polyubiquitinated proteins from TUBEs

Up to 1000-fold higher affinity than the single UBA domain

Avoid overexpression of affinity-tagged ubiquitins for pull downs

Protect polyubiquitin chains without inhibitors specific to either the proteasome or deubiquitylase activity

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs protect polyubiquitinated proteins from deubiquitination and proteasomal degradation, allowing for detection at relatively low abundance. These properties effectively allow TUBEs to “capture” protein in its polyubiqutinated state. TUBEs can be used to isolate, enrich, and identify ubiquitinated proteins from cell and tissue extracts. Agarose coupled TUBEs allow for efficient recovery of polyubiquitinated proteins in a single step. Polyubiquitinated protein can easily be separated from TUBEs for proteomic studies or identification by Western blotting. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply with these terms.

Info

Tag	His6-SUMO
Molecular Weight	N/A
Physical State	Liquid
Quantity	1 mL
Concentration	Variable
Storage	-20°C, avoid freeze/thaw cycles, avoid storing below this temperature