UM101-200µg Anti-Ub Tube1, GST

UM402: TUBE 2 (Agarose)

$555.00

In stock

SKU: UM-0402-1000 Category: Tags: , ,

Specifications

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” protein in its polyubiquitin state. Agarose coupled TUBEs allows for efficient recovery of polyubiquitinated proteins in a single step. Polyubiquitinated protein can easily be separated from TUBEs for proteomic studies or for identification by western blotting.

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Info

Tag N/A
Molecular Weight
Physical State Liquid
Quantity 1 mL
Concentration Variable
Storage -20°C, avoid freeze/thaw cycles, avoid storing below this temperature

Additional information

SKU

Cat #

UM402

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Applications

  • Pull down of polyubiquitylated proteins from cell lines, tissues and organs
  • Isolation of polyubiquitinated proteins for proteomic studies
  • Protection of polyubiquitylated proteins from deubiquitylation and degradation by the proteasome

Benefits

  • One step recovery of polyubiquitinated proteins
  • Efficient isolation of polyubiquitinated proteins from TUBEs
  • Up to 1000-fold higher affinity than the single UBA domain
  • Avoid overexpression of affinity-tagged ubiquitins for pull downs
  • Protect polyubiquitin chains without inhibitors specific to either the proteasome or deubiquitylase activity

Documents