Questions about a product, technology or experimental design? Give us a call 610-644-8845
Questions about a product, technology or experimental design? Give us a call 610-644-8845.

UM420: Ubiquitin Mass Spectrometry Kit

The LifeSensors UM420 Ubiquitin Mass Spectrometry Kit is designed for comprehensive proteomic analysis of polyubiquitinated proteins, facilitating the identification of ubiquitination sites and the profiling of the ubiquitome. By integrating TUBE (Tandem Ubiquitin Binding Entities) technology with mass spectrometry, this kit enables the enrichment of ubiquitinated proteins from complex biological samples without the need for labeling. Applications include the discovery of novel E3 ligases and their substrates, mechanistic studies of ubiquitin signaling, and the evaluation of small-molecule drug candidates such as PROTACs and molecular glues. The UM420 kit is a valuable tool for advancing research in targeted protein degradation and biomarker discovery.

$1,590.00

Mass spectrometry (MS) proteomics, especially when combined with TUBE technologies for protein enrichment, can be used in several innovative and impactful applications. Quantitatively profiling the ubiquitome can lead to the identification of ubiquitination sites on target proteins, and by extension unveiling the mechanisms of undiscovered E3 ligases with their substrates. MS can also inform small molecule drug discovery for these targets (e.g. PROTACs, molecular glues) by quantitatively reporting on how much target protein is degraded in the presence or absence of a drug candidate. This approach can also greatly aid the discovery and development of novel diagnostic biomarkers for challenging disease targets and development of clinically monitoring disease progression. All these applications require a simple, label-free, rapid ubiquitome approach. This kit is designed to address this for rapid yet reproducible proteomics. *The plate is designed for research use only. This product has been utilized externally in a K48 format for use in plants.

Ubiquitin Mass Spectrometry Kit Components:

Mammalian Cell Lysis Buffer: 5 mL Lysis Buffer, (contains protease inhibitors) (Store at -80°C)
UPS Inhibitor Cocktail: 50 µL, 100X (Store at -80°C)
Magnetic TUBE Reagent: 10 reactions (10 vials) (Store at 4°C)
Decomplexing Reagent 50µL, 100X (Store at -80°C)
TUBE Wash Reagent: 1000 µL, 10 reactions (Store at 4°C)
TUBE Elution Reagent: 300 µL, 10 reactions (Store at 4°C)
TUBE Neutralization Reagent: 100 µL, 10 reactions (Store at 4°C)
Digestion Buffer: 5 mL, 10 reactions (Store at 4°C)
Reducing solution: 1000 µL, 10 reactions (Store at -80°C)
Alkylating solution: 1000 µL, 10 reactions (Store at -80°C)
Trypsin solution: 20 µg, 10 reactions (Store at -80°C)
Gel Wash Buffer: 20mL, 10 reactions (Store at -80°C)
  Need guidance or technical assistance with LifeSensors’ PROTAC Assay Kit and Plate? We’re happy to help! Contact our technical support team at customerservice@lifesensors.com. Learn more about PROTACs here.

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

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PROTAC is a registered trademark of Arvinas Operations, Inc., and is used under license.

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