UM102: TUBE 2 (GST)

LifeSensors UM102 is a GST-tagged Tandem Ubiquitin Binding Entity (TUBE 2) designed for high-affinity capture and stabilization of polyubiquitylated proteins in research applications.

$603.00 – $1,853.00

Product Advantages

Pull down of poly-ubiquitylated proteins from cell and tissue lysates

Protection of poly-ubiquitylated proteins from both deubiquitylation and degradation by the proteasome

Benefits

Up to 1000-fold higher affinity than the single UBA domain

Avoid overexpression of affinity-tagged ubiquitins for pull downs

Protect poly-ubiquitin chains without inhibitors specific to the proteasome or deubiquitylase activity

Replace ubiquitin specific antibodies for enrichment of poly-ubiquitylated proteins

Based on UBA domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs have been shown to have a protective effect on polyubiquitinated proteins from both deubiquitination and proteasome-mediated degradation, allowing for detection of relatively low abundance proteins that cannot be detected with current technologies. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Info

Tag	GST
Molecular Weight	56 kDa
Physical State	Liquid
Quantity	200 µg and 1 mg
Concentration	Variable
Storage	-80°C, avoid freeze/thaw cycles