UM306: M1 TUBE (Biotin)

LifeSensors UM306 is a biotinylated M1-TUBE reagent with high affinity and selectivity for linear (M1-linked) polyubiquitin chains, used to isolate and study M1-polyubiquitinated proteins in cell and tissue extracts. Ideal for proteomics and ubiquitin pathway research.

$707.00

SKU UM-0306-0050 Categories M1 TUBE1, TUBEs for Detection, TUBEs: Ubiquitin Affinity Matrix Tags Linkage-specific, Pull down, Tandem Ubiquitin Binding entities

In stock

Product Advantages

Applications

Isolation and enrichment of M1-polyubiquitinated proteins from cell and tissue extracts
Isolation of ubiquitylated proteins for proteomic studies

Benefits

Nanomolar dissociation constant (Kd) for M1-chains
1000 to 10,000-fold preference for M1 chains over K48- or K63- chains
Overexpression of epitope-tagged ubiquitin for pull downs is not necessary
Compatible with Ni+2IMAC, streptavidin, or FLAG® -based isolation technologies, providing flexibility and specificity

LifeSensors has developed TUBE (Tandem Ubiquitin Binding Entity) technology for the detection, characterization and isolation of polyubiquitylated proteins from cells and tissue extracts. TUBEs capitalize on the linkage of multiple ubiquitin interacting motifs (UIMs) to generate reagents with a high affinity for polyubiquitin. TUBEs have 100 to 1000-fold higher affinity for polyubiquitin chains compared to monomer ubiquitin binding domains (UBDs). TUBEs both stabilize and bind to ubiquitylated proteins, serving as an indispensable tool for ubiquitologists. Our M1 (linear) TUBE has high selectivity for M1-linked polyubiquitin over the more common K48- and K63-linked polyubiquitin chains, making it a powerful tool for studying the biological consequences of this ubiquitin linkage type. This product is protected by one or more US or Foreign patents.

Info

Tag	Biotin
Molecular Weight	33.4 kDa
Physical State	Liquid
Quantity	50 µg
Concentration	Variable
Storage	-80°C, avoid freeze/thaw cycles

Datasheet

Datasheet

Applications

Isolation and enrichment of M1-polyubiquitinated proteins from cell and tissue extracts
Isolation of ubiquitylated proteins for proteomic studies

Benefits

Nanomolar dissociation constant (Kd) for M1-chains
1000 to 10,000-fold preference for M1 chains over K48- or K63- chains
Overexpression of epitope-tagged ubiquitin for pull downs is not necessary
Compatible with Ni+2IMAC, streptavidin, or FLAG® -based isolation technologies, providing flexibility and specificity