UC104: E2 SELECT Profiling & Selection Kit

SKU UC-0104-0096 Categories E1-E2-E3 Assays, Kits and Plates, Research Kits

E2 SELECT™ Kit: Identifies optimal E2 enzyme for your E3 ligaseion. Uses plates pre-coated with polyUb-binding reagent + 24 E2 enzymes. Add ATP + E3 → polyubiquitination occurs. Detects chains via chemiluminescent ELISA. Patented; purchase requires Limited Use License compliance.

$782.00

In stock

The E2 SELECT™ Profiling & Selection Kit allows for assessing the E2-E3 functional relationship. Therefore, it allows for identifying the optimal E2-conjugating enzyme for an E3 ligase of interest. The assay is based on the use of a plate that is pre-coated with a proprietary polyubiquitin-capture reagent. The E1 enzyme and ubiquitin are provided in the plate along with twenty-four E2 enzymes. Addition of ATP and of the E3 ligase of interest initiates the polyubiquitination process. The resulting polyubiquitin chains are captured in the wells and are detected in a chemiluminescent “sandwich” ELISA-like assay. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Kit Components:

1 x pre-coated 96-well plate containing a mix of E1, E2, and Ubiquitin. 24 E2s are provided in triplicate.
E1-E2-E3 Control
Linear Polyubiquitin Detection Control
Detection Reagent 1

Info

Storage	Store at -80°C
Stability	Each plate is individually prepared. Use the kit within one month.

Applications

Testing functionality of over 24 E2’s with an E3 ligase of interest
Ready-to-assay. Contains 24 human E2’s pre-plated in triplicate with E1 and Ubiquitin.
Detection system provides robust readout for E3 ligase activity.
E2 SELECT™ Kit utilizes non-radioactive reporter substrates.
Uses native, rather than tagged, ubiquitin

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

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UC104: E2 SELECT Profiling & Selection Kit

Kit Components:

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