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UE905: UbiQuant™ Ultra Plate

$345.00

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SKU: UE-0905 Category:

Specifications

The UbiQuant™ Ultra assay is a robust tool for assessing the poly-ubiquitination levels of low abundant protein of interest in cell lysate. This platform is based on capturing poly-ubiquitinated proteins on the plate followed by detection of the protein of interest (either native or tagged) using an antibody for the purpose of quantifying the poly-ubiquitinated forms of the protein. Detecting changes in the ubiquitination state of specific proteins in response to external stimuli has been a long and difficult process involving immunoprecipitation followed by Western blot analysis. The UbiQuant™ S kit is a quick, robust, and quantitative alternative to IP/WB analysis.

Note: This product only includes the coated plate. See UbiQuant S Kit for a complete experiment (plate, antibodies, detection reagent, blocking agent).

Storage

  • Long term: -80 C for up to 1 year
  • Short term: 4 C for up to 4 weeks

Additional information

Plate Size

96-well plate, 384-well plate

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Applications

 

  • Determine changes in the level of substrate poly-ubiquitination as a result of cell treatment.
  • Uses an antibody that specifically binds to the protein of interest.
  • High throughput, quantitative method

 

 

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