UB213: UBE2E3

LifeSensors UB213 is a purified human UBE2E3 enzyme, an E2 ubiquitin-conjugating enzyme used in ubiquitination research to study protein regulation and degradation pathways.

$245.00 – $487.00

SKU UB-0213 Categories E2 Ubiquitin Conjugating Enzymes, Ubiquitin Ligases and Enzymes

Product Advantages

Applications

Identify cognate E2/E3 pairs
Characterize upregulation/downregulation of E2 enzymes
Build poly-ubiquitin chains
Characterize ubiquitin chain specificity of E2 enzymes
Analyze expression of E2 enzymes

Benefits

Untagged (native) E2 conjugation enzyme

UBE2E3 is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. The deduced 207-amino acid protein shares over 94% amino acid identity with the UBC domains of class III E2s, UbcH6, UBE2E2, UbcM2, UbcM3, and UbcD2. Of these, however, only UbcM2 (thought to be the mouse homolog) exhibits homology in the N-terminal extension. UBE2E3 forms a thioester bond with ubiquitin in an E1-dependent manner and mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase.

Info

Species	Human
Source	E. coli
Tag	None
Molecular Weight	22,913 Da
Quantity	0.8 nmol, 3.0 nmol
Concentration	40 µM
Formulation	20 mM Tris pH 7.4, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage	-80°C, avoid freeze/thaw cycles