UC105: E1 LITE – UBE1 Activity Assay Kit


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The E1 LITE – UBE1 Activity Assay is a kit for assessing E1 enzyme activity. This plate-based assay is based on the transfer of FRET energy between the acceptor-charged ubiquitin and the donor-charged ubiquitin as they both bind to the E1 enzyme. The assay employs terbium as the donor fluorophore, which has a long-lived fluorescence allowing for a time-delayed reading of the FRET signal, or time-resolved FRET (TR-FRET). This reduces the contribution from buffers, proteins, and chemical compounds.

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Additional information

Plate Size(*)

96-well plate, 384-well plate


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  • Screening for inhibitors of the ubiquitin E1 enzyme, UBE1
  • Characterization of UBE1 kinetics
  • Characterization of the mechanisms of E1 inhibitors
  • Secondary screening of E3 ligase, kinase, or other target inhibitors for inhibition of UBE1
  • Eliminates the need for radioactive or Western blot-based detection of E1-ubiquitin-thioester formation
  • Homogenous assay compatible with high-throughput screening for UBE1 inhibitors
  • Compatible with 96-well and 384-well formats