UC105: E1 LITE – UBE1 Activity Assay Kit

The E1 LITE – UBE1 Activity Assay is a kit for assessing E1 enzyme activity. This plate-based assay is based on the transfer of FRET energy between the acceptor-charged ubiquitin and the donor-charged ubiquitin as they both bind to the E1 enzyme. The assay employs terbium as the donor fluorophore, which has a long-lived fluorescence allowing for a time-delayed reading of the FRET signal, or time-resolved FRET (TR-FRET). This reduces the contribution from buffers, proteins, and chemical compounds.

This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.