UB205: Ubc13

LifeSensors UB205 is a Ubc13 E2 ubiquitin conjugating enzyme used in research to study ubiquitination pathways, protein regulation, and cellular processes.

$294.00 – $439.00

SKU UB-0205 Categories E2 Ubiquitin Conjugating Enzymes, Ubiquitin Ligases and Enzymes

Product Advantages

Identify cognate E2/E3 pairs
Characterize upregulation/downregulation of E2 enzymes
Build poly-ubiquitin chains
Characterize ubiquitin chain specificity of E2 enzymes
Analyze expression of E2 enzymes

Benefits

Untagged (native) E2 conjugation enzyme

Ubc13 is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. Ubc13 acts with the ubiquitin E2 variant (UEV) protein Mms2, forming a heterodimeric complex that assembles K63-linked polyubiquitin chains utilized in non-degradative ubiquitin signaling pathways (DNA repair, signal transduction). The Ubc13/Mms2 complex is unique in that it is has the ability to form free K63-linked polyubiquitin chains in solution in the absence of an E3.

Info

Species	S. cerevisiae
Source	E. coli
Tag	His₆
Molecular Weight	17 kDa
Quantity	0.8 nmol, 3.0 nmol
Concentration	40 µM
Formulation	50mM HEPES pH 7.5, 150 mM NaCl, 10% glycerol
Storage	-80°C, avoid freeze/thaw cycles