UBE2M (also known as Ubc12) is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. UBE2M is involved in the conjugation of the ubiquitin-like protein (Ubl) NEDD8 to target substrates (NEDDylation) along with the APPBP1/Uba3 activating enzyme. NEDDylation is required for cell cycle progression. In addition, NEDD8 is conjugated to cullin family members which are a part of SCF ubiquitin E3 ligase complexes, thereby activating them.
|Molecular Weight||21,894.2 Da|
|Quantity||0.8 nmol, 3.0 nmol|
|Formulation||50 mM Tris pH 7.5, 150 mM NaCl, 10 mM DTT, 10% glycerol|
|Storage||-80°C, avoid freeze/thaw cycles|