UB204H: UBE2K (UbcH1), His6

LifeSensors UB204H is a His6-tagged UBE2K (UbcH1) enzyme that forms free K48-linked polyubiquitin chains, essential for studying protein degradation and ubiquitination pathways

$210.00 – $417.00

SKU UB-204H Categories E2 Ubiquitin Conjugating Enzymes, Neurodegeneration Tools, Neuronal DUBs and Ligases, Ubiquitin and Neuro-degeneration, Ubiquitin Ligases and Enzymes

Product Advantages

Identify cognate E2/E3 pairs
Characterize upregulation/downregulation of E2 enzymes
Build poly-ubiquitin chains
Characterize ubiquitin chain specificity of E2 enzymes
Analyze expression of E2 enzymes

Benefits

Untagged (native) E2 conjugation enzyme

UbcH1 (also known as E2-25K) is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. Once an E1 is carrying an activated ubiquitin in a ubiquitin-E1 thiolester complex, this activated ubiquitin is transferred to the active site cysteine of the E2 to form a ubiquitin-E2 thiolester complex. With the aid of an E3, the activated ubiquitin is then targeted to a specific substrate lysine. UbcH1 is a biochemically important E2 due to its ability to form free K48-linked polyubiquitin chains in solution in the absence of an E3. This ability is attributed to UbcH1 possessing a C-terminal UBA domain, in addition to its E2 catalytic domain.

Info

Species	Human
Source	E. coli
Tag	His6
Molecular Weight	23,400.7 Da
Quantity	0.8 nmol, 3.0 nmol
Concentration	40 µM
Formulation
Storage	-80°C, avoid freeze/thaw cycles