UM502F: TUBE 2 (Fluorescein)

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitylated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” protein in its polyubiquitin state. FLR-TUBE2 has an average of three fluorescein moieties (Exc. = 490 nm, Emm. = 520 nm) attached to the fusion tag of TUBE 2 (Cat. No. UM202). Since the fluorophore is attached to the tag, it does not affect binding of the tandem ubiquitin binding domains to polyubiquitin chains. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Info

Tag	His6
Molecular Weight	39.9 kDa
Physical State	Liquid
Quantity	50 µg
Concentration	Variable
Storage	-80°C, avoid freeze/thaw cycles