UM500M: High Capacity Control Magnetic Beads (negative control)

LifeSensors UM500M is a high-capacity control magnetic bead used as a negative control in ubiquitin affinity assays, ensuring reliable and accurate experimental results.

$205.00

SKU UM-0500M-1000 Categories All, Magnetic TUBEs, Pan-Selective TUBEs, TUBEs: Ubiquitin Affinity Matrix

In stock

Product Advantages

Applications

Negative control for pull-down experiments
“Pre-clearing” of cell lysates prior to pull-down experiments
General recommendations: Pre-clear or use as a negative control for 1ml of cell lysate (3-5 mg/ml total protein) through incubation with 100µl of slurry for 1hr (4°C). Individual conditions may have to be optimized by the end user.

For use in “pre-clearing” cell lysates, as well as a negative control for the pulldown of polyubiquitinated proteins in conjunction with magnetic beads-conjugated TUBEs. Control magnetic beads are generated by blocking an activated matrix (identical to that used for TUBEs conjugation) with ethanolamine. Control magnetic beads (1 mL) are supplied as a slurry in PBS, pH 7.2.

Info

Tag	N/A
Molecular Weight
Physical State	Liquid
Quantity	1 mL
Concentration	Variable
Storage	+4°C, avoid storing below this temperature

Datasheet
SDS

Datasheet
SDS

Applications

Negative control for pull-down experiments
“Pre-clearing” of cell lysates prior to pull-down experiments
General recommendations: Pre-clear or use as a negative control for 1ml of cell lysate (3-5 mg/ml total protein) through incubation with 100µl of slurry for 1hr (4°C). Individual conditions may have to be optimized by the end user.