Determine changes in the level of substrate poly-ubiquitination as a result of cell treatment.
Uses an antibody that specifically binds to the protein of interest.
High throughput, quantitative method
The UbiQuant™ Ultra assay is a robust tool for assessing the poly-ubiquitination levels of low abundant protein of interest in cell lysate. This platform is based on capturing poly-ubiquitinated proteins on the plate followed by detection of the protein of interest (either native or tagged) using an antibody for the purpose of quantifying the poly-ubiquitinated forms of the protein. Detecting changes in the ubiquitination state of specific proteins in response to external stimuli has been a long and difficult process involving immunoprecipitation followed by Western blot analysis. The UbiQuant™ S kit is a quick, robust, and quantitative alternative to IP/WB analysis.
Note: This product only includes the coated plate. See UbiQuant S Kit for a complete experiment (plate, antibodies, detection reagent, blocking agent).
