Specifications
The UbiQuant™ S assay is a robust tool for assessing the poly-ubiquitination levels of a protein of interest in cell lysate. This platform is based on capturing poly-ubiquitinated proteins on the plate followed by detection of the protein of interest (either native or tagged) using an antibody for the purpose of quantifying the poly-ubiquitinated forms of the protein. Detecting changes in the ubiquitination state of specific proteins in response to external stimuli has been a long and difficult process involving immunoprecipitation followed by Western blot analysis. The UbiQuant™ S kit is a quick, robust, and quantitative alternative to IP/WB analysis.
New assay plate developed for colorimetric detection allows for quantitative measurement using absorbance-based plate readers. Colorimetric readouts (650nm, 480nm) are more consistent day-to-day than luminescent-based detection methods, fast, and easy to use.
Note: This kit has been optimized and validated for assessing the poly-ubiquitination levels of tagged proteins. Therefore, the end-user must specify the epitope tag in order to receive the appropriate antibody for detection ( myc, HA, FLAG®, V5, GST. Unfortunately, His6 cannot be used for this assay).
UbiQuant™ S Assay Kit Components:
| Coated plate: | One pre-coated and blocked 96-well strip plate (Store at -80°C) |
| Empty strip plate frame: | One empty strip plate frame is provided |
| Primary Antibody (PA1) | Anti-epitope mAb in PBS Store at 4°C) |
| Colorimetric reagent (CR): | 5.0 mL vial of DR1 reagent (Store at 4°C) |
| Stopping reagent (SR): | 5.0 mL vial of DR2 reagent (Store at 4°C) |
| Blocking concentrate (BC): | 12 mL BC agent (Store at 4°C) |
| Detection Antibody (DA1): | 200X anti-mouse HRP conjugate |
| Plastic Plate Seals: | Four Plate Seals provided |
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