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UC106: E1/E2 LITE – Ubiquitin Transfer Assay Kit
LifeSensors UC106 is an E1/E2 LITE Ubiquitin Transfer Assay Kit designed for screening and characterizing inhibitors of UBE1 and UBE2D3 enzymes, enabling kinetic and mechanistic studies in ubiquitin research. Ideal for drug discovery and biochemical assays
Counterscreening hits from E3 Ligase screens for activity against representative E1 and E2 enzymes (UBE1 and UBE2D3)
Screening for inhibitors of UBE1 and UBE2D3
Characterization of the kinetics of E2 thioester formation
Characterization of the mechanism of E1 or E2 inhibition
Eliminates the need for radioactive or Western blot-based detection of E2 thioester formation
Homogenous assay compatible with high-throughput screening
Robust assay with a S/B >10 and a Z’ >0.7
Compatible with 96-well and 384-well formats
The E1/E2 LITE – Ubiquitin Transfer Assay is a kit for assessing E1/E2 ubiquitin transfer activity. This plate-based assay is based on the transfer of FRET energy between the acceptor-charged ubiquitin to the donor-charged E2 enzyme upon binding of the two. The assay employs terbium as the donor fluorophore, which has a long-lived fluorescence allowing for a time-delayed reading of the FRET signal, or time-resolved FRET (TR-FRET). This reduces the contribution from buffers, proteins, and chemical compounds.
This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.
