UB332: VHL/ELOB/ELOC Complex

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Specifications

Von Hippel-Lindau, or VHL, is an E3 Ligase that mediates ubiquitination and degradation of target proteins. VHL is seen to play a large role in tumor suppression, particularly due to its interactions with HIF. VHL can be used as the required E3 ligase in PROTAC (Proteolysis Targeting Chimera) drug design, protein degradation studies and selectivity profiling.

The VHL complex is a multi-subunit ubiquitin ligase constituted with VHL, Elongin B, Elongin C, Cullin-2 and Rbx1. Elongin B (ELOB) and Elongin C (ELOC) are heterodimers that bind to the BC-box motif present in SOCS- &VHL-box protein families. VHL is the substrate recognition component that brings target specificity.

In this specific E3 ligase complex, the ELOB/ELOC works as an adapter between target proteins and Cullin-2/Rbx1 in VHL-box E3 ubiquitin ligases. This complex is ideal for studying PROTAC interactions with biophysical and HTRF assays to monitor the ternary complex. This complex has Flag tagged proteins that are suitable for pulldown assays.

VHL Complex, E3 Ligase Info

Species Human
Source HEK293
Tag See Datasheet
Molecular Weight VHL: 25 kDa, ELOB: 14 kDa, ELOC: 13 kDa
Quantity 10 µg or 50 µg
Concentration Variable
Formulation 40 mM Tris-HCL, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT
Storage -80°C, avoid freeze/thaw cycles

 

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Additional information

Weight

10 µg, 50 µg

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Application

  • Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
  • This complex is ideal for studying PROTAC interaction with biophysical and HTRF assays for monitoring ternary complex.