UC104C: E2 SELECT Profiling & Selection Kit (Colorimetric)

The E2 SELECT™ Profiling & Selection Kit allows for assessing the E2-E3 functional relationship. Therefore, it allows for identifying the optimal E2-conjugating enzyme for an E3 ligase of interest. The assay is based on the use of a plate that is pre-coated with a proprietary polyubiquitin-capture reagent. The E1 enzyme and ubiquitin are provided in the plate along with twenty-four E2 enzymes. Addition of ATP and of the E3 ligase of interest initiates the polyubiquitination process. The resulting polyubiquitin chains are captured in the wells and are detected in a chemiluminescent “sandwich” ELISA-like assay. New assay plate developed for colorimetric detection allows for quantitative measurement using absorbance-based plate readers. Colorimetric readouts are more consistent day-to-day than luminescent-based detection methods, fast, and easy to use. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Kit Components:

• 1 x pre-coated 96-well plate containing a mix of E1, E2, and Ubiquitin. 24 E2s are provided in triplicate
• Linear Polyubiquitin Detection Control
• Detection Reagent 1
• Colorimetric Reagent
• Stop Solution

Info

Storage	Store at -80°C
Stability	Each plate is individually prepared. Use the kit within one month.