UB320: Parkin R275W Mutant E3 Ligase

Encoded by the PARK2 gene, the E3 ligase Parkin is part of the multi-protein E3 complex that encodes substrate proteins for degradation in the Ubiquitin-Proteasome Pathway. The precise function of this protein is unknown; however, mutations in this gene are known to cause a familial form of Parkinson’s disease known as autosomal recessive juvenile Parkinson’s disease (AR-JP). Parkin is described to be necessary for mitophagy (autophagy of mitochondria). The R275W mutation has been shown to destabilize Parkin and disrupt interaction with the helix that mediates pUb binding.

Species	Human
Source	E. coli
Tag	His10-SUMO
Molecular Weight	64 kDa
Quantity	25 µg
Concentration	Variable
Formulation	20 mM Tris pH 7.5, 150 mM NaCl, 10% Glycerol
Storage	-80°C, avoid freeze/thaw cycles