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Questions about a product, technology or experimental design? Give us a call 610-644-8845.

UB226H: UBE2A, His6

LifeSensors UB226H is a His6-tagged UBE2A E2 ubiquitin-conjugating enzyme essential for substrate ubiquitylation in protein degradation and signaling research.

Price range: $189.00 through $375.00

UBE2A is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. UBE2A is a substrate for cyclin-dependent kinase 2 with phosphorylation of Ser120, dramatically increasing ubiquitin conjugating activity.  During the cell cycle, peak phosphorylation of UBE2A coincides with peak ubiquitylation of histone H2B. Ubiquitylated H2B stimulates methylation of lysine 4 of histone H3. UBE2A and B are also involved in DNA lesion repair via recruitment of alternative polymerases to DNA. UBE2A has been implicated in regulating the ubiquitylation of aggregating proteins along with UBE2K and UBE2D2.

Info

Species Human
Source E. coli
Tag His6
Molecular Weight 18,309.5 Da
Quantity 0.8 nmol, 3.0 nmol
Concentration 40 µM
Formulation 50 mM Tris pH 7.5, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Applications

  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

Benefits

  • Untagged (native) E2 conjugation enzyme

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

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