UB226H: UBE2A, His6

LifeSensors UB226H is a His6-tagged UBE2A E2 ubiquitin-conjugating enzyme essential for substrate ubiquitylation in protein degradation and signaling research.

$210.00 – $417.00

SKU UB-226H Categories E2 Ubiquitin Conjugating Enzymes, Ubiquitin Ligases and Enzymes Tag His6

Product Advantages

Applications

Identify cognate E2/E3 pairs
Characterize upregulation/downregulation of E2 enzymes
Build poly-ubiquitin chains
Characterize ubiquitin chain specificity of E2 enzymes
Analyze expression of E2 enzymes

Benefits

Untagged (native) E2 conjugation enzyme

UBE2A is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. UBE2A is a substrate for cyclin-dependent kinase 2 with phosphorylation of Ser120, dramatically increasing ubiquitin conjugating activity. During the cell cycle, peak phosphorylation of UBE2A coincides with peak ubiquitylation of histone H2B. Ubiquitylated H2B stimulates methylation of lysine 4 of histone H3. UBE2A and B are also involved in DNA lesion repair via recruitment of alternative polymerases to DNA. UBE2A has been implicated in regulating the ubiquitylation of aggregating proteins along with UBE2K and UBE2D2.

Info

Species	Human
Source	E. coli
Tag	His₆
Molecular Weight	18,309.5 Da
Quantity	0.8 nmol, 3.0 nmol
Concentration	40 µM
Formulation	50 mM Tris pH 7.5, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage	-80°C, avoid freeze/thaw cycles