SI0102-5µg (panel) Linear (Ub2) di-ubiquitin

SI6304B: Biotinylated K63-Linked Tetra-Ubiquitin

LifeSensors SI6304B is a biotinylated K63-linked tetra-ubiquitin, ideal for research on ubiquitin signaling, protein regulation, and the ubiquitin-proteasome pathway.

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Product Advantages

Biotinylated chains are meant to label the protein so they can be selectively separated via a multitude of methods such as Western Blotting, pull downs (see website), ELISAs, etc.
Can use streptavidin or avidin to visualize this protein specifically.
Investigation of ubiquitin chain specificity and selectivity
Studies on the role of ubiquitin chains in protein degradation pathways (e.g., proteasomal and autophagic degradation)
Analysis of ubiquitin-mediated signaling pathways and cellular responses
Structural studies to elucidate the architecture and dynamics of ubiquitin chains
Screening assays to identify modulators of ubiquitin chain assembly and disassembly processes

K63 chains traditionally play a role in intracellular signaling, trafficking, and autophagy. The topology of this linkage and its apparent role in cellular processes are quite different from that of K48. Poly-Ub chains of this type appear to play a role in endocytic trafficking, DNA repair, neurodegeneration and more. K63 ubiquitin is formed via an isopeptide bond between Lysine 63 and the C-terminal Glycine. This linkage takes place onto other ubiquitin monomers and are then enzymatically linked from specific UPS enzymes. Once the K63 Tetra Ubiquitin has been formed we then biotinylate it once on one available site on the ubiquitin chain. This biotin then acts as a means of detection via streptavidin or vidin. The applications are numerous from Western Blots to ELISAs. Testing can vary based off intended application (EX: DUB activity/specificity). Info

Species	Human
Source	E. coli
Tag	Biotin
Molecular Weight	34759 Da (depending on degree of phosphorylation)
Quantity	Variable
Concentration	Variable
Formulation	20 mM Tris pH 7.5, 150 mM NaCl
Storage	-80°C, avoid freeze/thaw cycles

Document 1

Document 1

Biotinylated chains are meant to label the protein so they can be selectively separated via a multitude of methods such as Western Blotting, pull downs (see website), ELISAs, etc.
Can use streptavidin or avidin to visualize this protein specifically.
Investigation of ubiquitin chain specificity and selectivity
Studies on the role of ubiquitin chains in protein degradation pathways (e.g., proteasomal and autophagic degradation)
Analysis of ubiquitin-mediated signaling pathways and cellular responses
Structural studies to elucidate the architecture and dynamics of ubiquitin chains
Screening assays to identify modulators of ubiquitin chain assembly and disassembly processes