SI0604BP: Biotinylated K6-Linked Tetra-Ubiquitin (Phosphorylated)

K6-linked ubiquitination is traditionally associated with mitophagy and also a main contributor to the DNA damage response.  These chain types are also involved in protein stabilization and other non-degradative processes. Phospho-ubiquitin chains represent a specialized class of polyubiquitin characterized by phosphorylation at Serine 65 and play a central role in mitophagy signaling pathways. K6 Tetra-Ubiquitin (phosphorylated) is a tetrameric chain of wild-type ubiquitin, wherein ubiquitin monomers are enzymatically linked together via an isopeptide bond between Lysine 6 and the C-terminal Glycine. This linkage takes place onto other ubiquitin monomers and are then enzymatically linked from specific UPS enzymes. Once the K6 Tetra Ubiquitin has been formed we then enzymatically phosphorylated at the Ser65 position. Once phosphorylation is complete they are biotinylated once on one available site on the ubiquitin chain. This biotin then acts as a means of detection via streptavidin or avidin. The applications are numerous from Western Blots to ELISAs. Testing can vary based off of intended application (EX: DUB activity/specificity).   Info

Species	Human
Source	E. coli
Tag	Biotin
Molecular Weight	34842-35082 Da (depending on degree of phosphorylation)
Quantity	25µg
Concentration	Variable
Formulation	20 mM Tris pH 7.5, 150 mM NaCl
Storage	-80°C, avoid freeze/thaw cycles