SI0102-5µg (panel) Linear (Ub2) di-ubiquitin

SI240: Ubiquitin- Vinylmethyl ester

LifeSensors SI240 is a ubiquitin vinylmethyl ester (Ub-VME) probe used for studying deubiquitinating enzymes, enabling precise enzyme activity analysis in research.

$376.00

SKU SI-0240-0050 Categories Ubiquitin Chains and Derivatives, Ubiquitin Derivatives

In stock

Product Advantages

This inhibitor is useful for labeling DUBs in situ as well as preserving the integrity of polyubiquitin chains on modified proteins for analysis or purification.

Ub-VME is synthesized by the conjugation of 4-amino-but-2-enoic methyl ester to the C-terminus of UbiquitindeltaG76. Binding of ubiquitin by deubiquitylases (DUBs), for instance UCH’s and most USPs, positions the reactive vinyl bond next to the sulfhydryl-group of the active site cysteine. Nucleophilic attack by the sulfhydryl on the vinyl bond produces a stable, covalent thioether bond between Ubiquitin and the deubiquitylase. Thus Ub-VME is a potent suicide inhibitor of DUBs.

Info

Species	Human
Source	E. coli
Tag	None
Molecular Weight	8,604.9 Da
Quantity	50 µg
Concentration	Variable
Formulation	Lyophilized powder
Storage	-80°C, avoid freeze/thaw cycles

Datasheet

Datasheet

This inhibitor is useful for labeling DUBs in situ as well as preserving the integrity of polyubiquitin chains on modified proteins for analysis or purification.