SI0104: Linear Tetra-Ubiquitin

LifeSensors SI0104 is a linear tetra-ubiquitin chain used in research to study protein regulation and ubiquitin pathway mechanisms in cellular processes.

$320.00

SKU SI-0104-0100 Categories Ubiquitin Chains, Ubiquitin Chains and Derivatives

In stock

Product Advantages

Applications

Investigation of binding interactions through pull-down studies.

Determining potential deubiquitylase activity towards linear chains.

A wide range of cellular processes are modulated through the generation and attachment of polyubiquitin (polyUb) chains to target proteins. Increasing evidence suggests that polyUb chains joined through linear peptide bonds between the C-terminus of a ubiquitin and the N-terminus of another play important functional roles. The enzyme machinery responsible for the generation of linear polyUb chains has been termed LUBAC, consisting of HOIL-1L and HOIP. Chains of these type have an open conformation, similar to polyUb K63, but with very distinct functional properties. Linear polyUb chains are cleaved by the deubiquitylases CYLD, USP5 (IsoT), USP2 and have been shown to bind to many UBDs including NEMO and Trabin-n (3xnzf). Recombinant tetraubiquitin expressed as a linear chain. Amide linkages join the N- and C-terminus of each ubiquitin molecule to each other. This molecule is His-tagged at the N-terminus of the most distal ubiquitin.

Info

Species	Human
Source	E. coli
Tag	His₆
Molecular Weight	34,617.5 Da
Quantity	100 µg
Concentration	Variable
Formulation	20 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA
Storage	-80°C, avoid freeze/thaw cycles